Inherent genomic properties underlie the epigenomic heterogeneity of human induced pluripotent stem cells

横林, しほり, 藪田, 幸宏, 中川, 誠人, 沖田, 圭介, Hu, Bo, 村瀬, 佑介, 中村, 友紀, Bourque, Guillaume, Majewski, Jacek, 山本, 拓也, 斎藤, 通紀

doi:10.1016/j.celrep.2021.109909

Human induced pluripotent stem cells (hiPSCs) show variable differentiation potential due to their epigenomic heterogeneity, whose extent/attributes remain unclear, except for well-studied elements/chromosomes such as imprints and the X chromosomes. Here, we show that seven hiPSC lines with variable germline potential exhibit substantial epigenomic heterogeneity, despite their uniform transcriptomes. Nearly a quarter of autosomal regions bear potentially differential chromatin modifications, with promoters/CpG islands for H3K27me3/H2AK119ub1 and evolutionarily young retrotransposons for H3K4me3. We identify 145 large autosomal blocks (≥100 kb) with differential H3K9me3 enrichment, many of which are lamina-associated domains (LADs) in somatic but not in embryonic stem cells. A majority of these epigenomic heterogeneities are independent of genetic variations. We identify an X chromosome state with chromosome-wide H3K9me3 that stably prevents X chromosome erosion. Importantly, the germline potential of female hiPSCs correlates with X chromosome inactivation. We propose that inherent genomic properties, including CpG density, transposons, and LADs, engender epigenomic heterogeneity in hiPSCs.

Inherent genomic properties underlie the epigenomic heterogeneity of human induced pluripotent stem cells

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収録刊行物

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Inherent genomic properties underlie the epigenomic heterogeneity of human induced pluripotent stem cells

説明

収録刊行物

被引用文献 (2)*注記

参考文献 (85)*注記

関連プロジェクト

キーワード

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