Phase-Modulation Fluorometer Using a Phase-Modulated Excitation Light Source

DOI IR 28 References Open Access

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Description

We propose a phase-modulation fluorometer (PMF) with a light-emitting diode (LED) or a laser diode (LD) used as an excitation light source (ELS) that is driven in the phase-modulation (PM) mode. The PM-ELS generates many frequency sidebands that spread in the vicinity of carrier frequency fc with the interval of modulation frequency fm depending on the maximum phase deviation Δφ . The scheme enables us to derive fluorescence lifetime values of a multicomponent sample at one time. We show a typical numerical simulation result for explaining the principle of operation. To demonstrate the effectiveness of the proposed PMF, we have measured fluorescence lifetimes of three kinds of inorganic fluorescent glasses and that of a mixture solution of 1×10−6M rhodamine 6G and 1×10−6M coumarin 152 in ethanol with a volume ratio of 1 :1.

Journal

  • Optical Review

    Optical Review 19 (4), 222-227, 2012-07-31

    Springer Science and Business Media LLC

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