Induction of pluripotent stem cells from mouse embryonic and adult fibroblasts cultures by defined factors
書誌事項
- タイトル別名
-
- Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
- Induction of pluripotent stem cells from mouse embryonic and adult fibroblasts by defined factors
- Induction of pluripotent stem cells from mouse embryonic, adult fibroblast cultures by defined factors
- Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors [Journal Article]
- Induction of pluripotent stem cells from mouse embryonic and adult fibroblastic cultures by defined factors
- 公開日
- 2006-08-25
- 資源種別
- journal article
- 権利情報
-
- Copyright © 2006 Elsevier Inc. All rights reserved.
- This posting is granted exceptional permission by the owner of copyright.
- DOI
-
- 10.1016/j.cell.2006.07.024
- 公開者
- Elsevier Inc.
この論文をさがす
説明
Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was dispensable. These cells, which we designated iPS (induced pluripotent stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors.
収録刊行物
-
- Cell
-
Cell 126 (4), 663-676, 2006-08-25
Elsevier Inc.
- Tweet
キーワード
- Adult
- Animals
- Cell Differentiation/physiology
- Cell Transplantation
- Cells, Cultured
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism
- Embryo, Mammalian/cytology
- Embryo, Mammalian/physiology
- Fibroblasts/cytology
- Gene Expression Profiling
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism
- Humans
- Kruppel-Like Transcription Factors/genetics
- Kruppel-Like Transcription Factors/metabolism
- Mice
- Mice, Nude
- Mice, Transgenic
- Octamer Transcription Factor-3/genetics
- Octamer Transcription Factor-3/metabolism
- Oligonucleotide Array Sequence Analysis
- Pluripotent Stem Cells/cytology
- Pluripotent Stem Cells/physiology
- Proto-Oncogene Proteins c-myc/genetics
- Proto-Oncogene Proteins c-myc/metabolism
- SOXB1 Transcription Factors
- Trans-Activators/genetics
- Trans-Activators/metabolism
詳細情報 詳細情報について
-
- CRID
- 1050564285690822016
-
- NII論文ID
- 80018983952
- 20000893223
-
- NII書誌ID
- AA00600003
-
- ISSN
- 10974172
- 00928674
- http://id.crossref.org/issn/00928674
- https://id.crossref.org/issn/00928674
-
- HANDLE
- 2433/159777
-
- PubMed
- 32165579
-
- 本文言語コード
- en
-
- 資料種別
- journal article
-
- データソース種別
-
- IRDB
- Crossref
- CiNii Articles
- KAKEN
- OpenAIRE
