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Quantitative analysis of recombination between YFP and CFP genes of FRET biosensors introduced by lentiviral or retroviral gene transfer.
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Description
Biosensors based on the principle of Förster (or fluorescence) resonance energy transfer (FRET) have been developed to visualize spatio-temporal dynamics of signalling molecules in living cells. Many of them adopt a backbone of intramolecular FRET biosensor with a cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) as donor and acceptor, respectively. However, there remains the difficulty of establishing cells stably expressing FRET biosensors with a YFP and CFP pair by lentiviral or retroviral gene transfer, due to the high incidence of recombination between YFP and CFP genes. To address this, we examined the effects of codon-diversification of YFP on the recombination of FRET biosensors introduced by lentivirus or retrovirus. The YFP gene that was fully codon-optimized to E.coli evaded the recombination in lentiviral or retroviral gene transfer, but the partially codon-diversified YFP did not. Further, the length of spacer between YFP and CFP genes clearly affected recombination efficiency, suggesting that the intramolecular template switching occurred in the reverse-transcription process. The simple mathematical model reproduced the experimental data sufficiently, yielding a recombination rate of 0.002-0.005 per base. Together, these results show that the codon-diversified YFP is a useful tool for expressing FRET biosensors by lentiviral or retroviral gene transfer.
Journal
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- Scientific reports
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Scientific reports 5 13283-, 2015-08-20
Nature Publishing Group
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Keywords
- Recombination, Genetic
- Genetic Vectors
- Green Fluorescent Proteins
- Lentivirus
- Gene Transfer Techniques
- Reproducibility of Results
- Biosensing Techniques
- Templates, Genetic
- Models, Theoretical
- Article
- Luminescent Proteins
- HEK293 Cells
- Retroviridae
- Bacterial Proteins
- Fluorescence Resonance Energy Transfer
- Humans
- Computer Simulation
- Codon
- HeLa Cells
Details 詳細情報について
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- CRID
- 1050564285769825152
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- NII Article ID
- 120005672592
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- ISSN
- 20452322
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- HANDLE
- 2433/201903
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- PubMed
- 26290434
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- IRDB
- Crossref
- CiNii Articles
- KAKEN
- OpenAIRE