Mechanical diagnosis of human eryhrocytes by ultra-high speed manipulation unraveled critical time window for global cytoskeletal remodeling
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- タイトル別名
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- Mechanical diagnosis of human erythrocytes by ultra-high speed manipulation unraveled critical time window for global cytoskeletal remodeling
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説明
Large deformability of erythrocytes in microvasculature is a prerequisite to realize smooth circulation. We develop a novel tool for the three-step "Catch-Load-Launch" manipulation of a human erythrocyte based on an ultra-high speed position control by a microfluidic "robotic pump". Quantification of the erythrocyte shape recovery as a function of loading time uncovered the critical time window for the transition between fast and slow recoveries. The comparison with erythrocytes under depletion of adenosine triphosphate revealed that the cytoskeletal remodeling over a whole cell occurs in 3 orders of magnitude longer timescale than the local dissociation-reassociation of a single spectrin node. Finally, we modeled septic conditions by incubating erythrocytes with endotoxin, and found that the exposure to endotoxin results in a significant delay in the characteristic transition time for cytoskeletal remodeling. The high speed manipulation of erythrocytes with a robotic pump technique allows for high throughput mechanical diagnosis of blood-related diseases.
収録刊行物
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- Scientific Reports
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Scientific Reports 7 43134-, 2017-02-24
Springer Nature
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詳細情報 詳細情報について
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- CRID
- 1050564285794504832
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- NII論文ID
- 120005980829
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- ISSN
- 20452322
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- HANDLE
- 2433/218417
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- PubMed
- 28233788
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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