Ligand-Mediated G-Quadruplex Induction in a Double-Stranded DNA Context by Cyclic Imidazole/Lysine Polyamide

HANDLE Open Access
  • Asamitsu, Sefan
    Department of Chemistry, Graduate School of Science, Kyoto University
  • Li, Yue
    Department of Chemistry, Graduate School of Science, Kyoto University
  • Bando, Toshikazu
    Department of Chemistry, Graduate School of Science, Kyoto University
  • Sugiyama, Hiroshi
    Department of Chemistry, Graduate School of Science, Kyoto University ・Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto University

Abstract

G-quadruplex (G4) DNA is often observed as a DNA secondary structure in guanine-rich sequences, and is thought to be relevant to pharmacological and biological events. Therefore, G4 ligands have attracted great attention as potential anticancer therapies or in molecular probe applications. Here, we designed cyclic imidazole/lysine polyamide (cIKP) as a new class of G4 ligand. It was readily synthesized without time-consuming column chromatography. cIKP selectively recognized particular G4 structures with low nanomolar affinity. Moreover, cIKP exhibited the ability to induce G4 formation of the promoter of G4-containing DNA in the context of stable double-stranded DNA (dsDNA) under molecular crowding conditions. This cIKP might be applicable as a molecular probe for the detection of potential G4-forming sequences in dsDNA.

Journal

  • ChemBioChem

    ChemBioChem 17 (14), 1317-1322, 2016-07-15

    Wiley-VCH Verlag

Keywords

Details 詳細情報について

  • CRID
    1050564285810894976
  • NII Article ID
    120006460112
  • ISSN
    14394227
  • HANDLE
    2433/230877
  • Text Lang
    en
  • Article Type
    journal article
  • Data Source
    • IRDB
    • CiNii Articles

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