Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions

HANDLE オープンアクセス
  • 池田, 俊太郎
    Laboratory of Animal Physiology and Functional Anatomy, Graduate School of Agriculture, Kyoto University
  • 杉本, 実紀
    NODAI Genome Research Center, Tokyo University of Agriculture
  • 久米, 新一
    Department of Animal Sciences, Tokyo University of Agriculture
  • Sugimoto, Miki
    Laboratory of Animal Physiology and Functional Anatomy, Graduate School of Agriculture, Kyoto University
  • Kume, Shinichi
    Laboratory of Animal Physiology and Functional Anatomy, Graduate School of Agriculture, Kyoto University

抄録

Methionine adenosyltransferase (MAT) is involved in folate-mediated one-carbon metabolism, which is essential for preimplantation embryos in terms of both short-term periconceptional development and long-term phenotypic programming beyond the periconceptional period. Here, our immunofluorescence analysis of bovine oocytes and preimplantation embryos revealed the consistent expression of MAT2A (the catalytic subunit of the ubiquitously expressed-type of MAT isozyme) during this period. Addition of the MAT2A inhibitor FIDAS to the culture media of bovine preimplantation embryos reduced their blastocyst development, revealing the particular importance of MAT2A in successful blastocyst development. Exploration of MAT2A-associated genomic regions in bovine blastocysts using chromatin immunoprecipitation and sequencing (ChIP-seq) identified candidate MAT2A-associated genes implicated not only in short-term periconceptional embryo development, but also in long-term phenotypic programming during this period in terms of growth, metabolism, and immune functions. These results suggest the critical involvement of MAT2A in the periconceptional period in life-long programming of health and disease as well as successful preimplantation development.

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キーワード

詳細情報 詳細情報について

  • CRID
    1050564285810937728
  • NII論文ID
    120006463796
  • ISSN
    20452322
  • HANDLE
    2433/231066
  • 本文言語コード
    en
  • 資料種別
    journal article
  • データソース種別
    • IRDB
    • CiNii Articles

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