Static and dynamic hypergravity responses of osteoblasts and osteoclasts in medaka scales

Yano, Sachiko, Kitamura, Kei-ichiro, Satoh, Yusuke, Nakano, Masaki, Hattori, Atsuhiko, Sekiguchi, Toshio, Ikegame, Mika, Nakashima, Hiroshi, Omori, Katsunori, Hayakawa, Kazuichi, Chiba, Atsuhiko, Sasayama, Yuichi, Ejiri, Sadakazu, Mikuni-Takagaki, Yuko, Mishima, Hiroyuki, Funahashi, Hisayuki, Sakamoto, Tatsuya, Suzuki, Nobuo

doi:10.2108/zsj.30.217

Fish scales are a form of calcified tissue similar to that found in human bone. In medaka scales, we detected both osteoblasts and osteoclasts and subsequently developed a new scale assay system. Using this system, we analyzed the osteoblastic and osteoclastic responses under 2-, 3-, and 4-gravity (G) loading by both centrifugation and vibration. After loading for 10 min, the scales from centrifugal and vibration loading were incubated for 6 and 24 hrs, respectively, after which the osteoblastic and osteoclastic activities were measured. Osteoblastic activity significantly increased under 2- to 4-G loading by both centrifugation and vibration. In contrast, we found that osteoclastic activity significantly decreased under 2- and 3-G loading in response to both centrifugation and vibration. Under 4-G loading, osteoclastic activity also decreased on centrifugation, but significantly increased under 4-G loading by vibration, concomitant with markedly increased osteoblastic activity. Expression of the receptor activator of the NF-αB ligand (RANKL), an activation factor of osteoclasts expressed in osteoblasts, increased significantly under 4-G loading by vibration but was unchanged by centrifugal loading. A protein sequence similar to osteoprotegerin (OPG), which is known as an osteoclastogenesis inhibitory factor, was found in medaka using our sequence analysis. The ratio of RANKL/OPG-like mRNAs in the vibration-loaded scales was significantly higher than that in the control scales, although there was no difference between centrifugal loaded scales and the control scales. Accordingly, medaka scales provide a useful model by which to analyze bone metabolism in response to physical strain. © 2013 Zoological Society of Japan.

Static and dynamic hypergravity responses of osteoblasts and osteoclasts in medaka scales

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Static and dynamic hypergravity responses of osteoblasts and osteoclasts in medaka scales

書誌事項

この論文をさがす

説明

収録刊行物

被引用文献 (6)*注記

参考文献 (38)*注記

関連プロジェクト

キーワード

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