ラット腎虚血再灌流モデルにおけるklothoの発現調節

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  • ラット ジンキョ ケツ サイカンリュウ モデル ニ オケル klotho ノ ハツゲン チョウセツ
  • Regulation of Klotho Expression in the Experimental Ischemic-Reperfusion Renal Injury Model

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A new gene, klotho, is associated with the suppression of several aging phenotypes. High expression of the klotho gene was detected in the kidney and several studies have reported the alternation of its expression level in some animal models and stressful conditions, so we explored the physiological relevance of the klotho expression in the kidney following ischemic-reperfusion renal injury (IRI). Male Wistar rats underwent bilateral renal ischemia or sham operation, followed by reperfusion for 1, 3 and 10 days. The expression of renal klotho mRNA and protein was assessed with real-time PCR or Western blotting. Creatinine levels were determined for functional parameters. Immunohistochemical and morphological studies were performed. Apoptotic cells were determined by TUNEL staining. Renal klotho mRNA and protein expression was significantly reduced in IRI rats the first day after ischemia. The number of klotho positive cells in the kidney 3 days after IRI was less than that in the sham-operated kidney. In addition, both TUNEL and klotho staining showed co-localization. The data indicated that the klotho gene is implicated in the pathophysiology associated with IRI. Downregulation of the renal klotho gene plays a role in the aggravation of ischemic acute renal failure.

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