Bibliographic Information
- Other Title
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- ARABIDOPSIS THALIANA ト キャベツ ノ タイサイボウ ザッシュ ノ サクシュツ オヨビ ソレラ ノ ゲノム コウゾウ
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Abstract
Somatic hybrids were produced in the combinations of A. thaliana and two varieties of cabbage (‘Fujiwase’ and ‘Chusei-Succession’). Protoplasts isolated from aseptically grown seedlings of the two parental species were fused under polyethylene glycol(PEG). The proto plasts were cultured in modified 8P liquid medium, and the micro-calli were transfered to callus development medium. After the transplanting of calli onto regeneration medium, shoots were regenerated with the frequencies of 12.4% (A. thaliana + ‘Fujiwase’) and 26.0% (A. thaliana + ‘Chusei-Succession’). Morphological observation and molecular studies demonstrated that the somatic hybrids were produced in both the combinations. The somatic hybrids between A. thaliana and ‘Fujiwase’ showed hybridity in the nucleus and the mitochondrial genomes, while those between A. thaliana and ‘Chusei-Succession’ possessed hybrid characteristics in the mitochondria. In contrast, all the hybrids contained only cabbage genome for chloroplasts. The hybrids are expected to be useful materials for breeding of cabbage.
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KJ00003145552
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研究論文
Paper
Journal
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- The bulletin of the Research Institute of Advanced Technology Kyoto Sangyo University
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The bulletin of the Research Institute of Advanced Technology Kyoto Sangyo University 4 15-19, 2005-07
[京都] : 京都産業大学先端科学技術研究所 ; 2002-2020
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Details 詳細情報について
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- CRID
- 1050564287372129152
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- NII Article ID
- 110001380263
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- NII Book ID
- AA11815191
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- HANDLE
- 10965/00002560
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- NDL BIB ID
- 7794951
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- ISSN
- 13473980
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- Text Lang
- ja
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- Article Type
- departmental bulletin paper
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- Data Source
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- IRDB
- NDL
- CiNii Articles
- KAKEN