Effects of Zn2+ chelators, DTPA and TPEN, and ZnCl2 on the cells treated with hydrogen peroxide: a flow-cytometric study using rat thymocytes
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Recently, we have revealed that trace Zn2+ partly attenuates Ca2+-dependent cell death induced by A23187, a calcium ionophore, in rat thymocytes. In this study, to see if Zn2+ attenuates the H2O2-induced cell death that is also Ca2+-dependent, the effects of ZnCl2 and chelators for Zn2+ have been examined by using a flowcytometer with propidium iodide. The incubation with H2O2 increased the cell lethality. Simultaneous application of ZnCl2 greatly augmented the H2O2-induced increase in lethality. DTPA, a chelator for extracellular Zn2+, did not affect the increase in cell lethality by H2O2. However, the H2O2-induced increase in cell lethality was greatly attenuated by TPEN, a chelator for extracellular and intracellular Zn2+. Taken together, it may be likely that intracellular Zn2+ modifies the H2O2-induced cytotoxicity. However, it cannot be ruled out the possibility that TPEN chelates intracellular Fe2+, resulting in inhibiting the formation of hydroxyl radical from H2O2 that leads to an attenuation of H2O2 cytotoxicity.
収録刊行物
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- 徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima
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徳島大学総合科学部自然科学研究 = Natural Science Research, The University of Tokushima 23 (2), 13-19, 2009-07-01
徳島大学総合科学部
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詳細情報 詳細情報について
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- CRID
- 1050564287417119872
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- NII論文ID
- 120002693581
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- NII書誌ID
- AN10065859
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- ISSN
- 09146385
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- NDL書誌ID
- 10783979
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- 本文言語コード
- en
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- departmental bulletin paper
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