Lipid Transfer Proteinに対するモノクローナル抗体を用いたリンゴ使用量の評価系の確立

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  • Lipid Transfer Protein ニ タイスル モノクローナル コウタイ オ モチイタ リンゴ シヨウリョウ ノ ヒョウカケイ ノ カクリツ
  • Evaluation of Apple Content with Monoclonal Antibodies Specific to Lipid Transfer Protein

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Abstract

In order to establish evaluation system for apple content in foods, monoclonal antibodies were raisedagainst apple lipid transfer protein (LTP). A sandwich-type of enzyme-linked immunosorbent assay (ELISA)was developed by using two of the five monoclonal antibodies obtained. This ELISA showed a practicalworking range of 0.03-10 ng/mL for apple LTP and no cross-reactivity with other fruits tested except plum.LTP could be determined with this ELISA in peel selectively and in foods with apple labeling such as applejuice, vegetable juice and even in curry roux.

identifier:http://repo.kyoto-wu.ac.jp/dspace/handle/11173/125

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