Studies on the Diffusion of the Electrolytes in Organism(I) : Determination of the Diffusion Constants of Acetylcholine Chloride in NaCl Solution

(1)筆者は,塩化アセチルコリンの拡散定数を,初濃度0.0181g/mlから0.0449g/mlの範囲で,また,塩効果のために0.35モルの塩化ナトリウムを添加して測定した。この場合,添加塩濃度を一定にして測定した。(2)それぞれの濃度の拡散係数は,Beckmann-Rosenbergの方法および細野-桜田の方法を用いて算出したが,k_Dの値が著じるしく大きくなった。(3)とくにBeckmann-Rosenbergの方法による濃度依存係数k_<DBR>は,初濃度の変化に対応して変化することが明らかになった。すなわち,D_C=D_0(AC√<C_0>+BC+1)で一般化でき,本実験の条件においては,D_C=D_0(225.49C√<C_0>-57.50C+1)が成立する。ただし,D_C:濃度Cに対する拡散係数,D_0:初濃度C_0の溶液の極限希薄濃度における拡散係数(拡散定数),A,B:物質によって定まる実験定数とする。

The diffusion constants of acetylcholine chloride in NaCl solution have been investigated in order to examine the behavior of the electrolytes in organism. The diffusion coefficients of this solution were measured in the initial concentration of 0.0181 g/ml, 0.0242g/ml and 0.0449g/ml by the method of Schlieren diagonal cylindrical lense. The amount of sodium chloride added for the salt-effect in the solution was kept at constant throughout these experiments. The diffusion coefficients were calculated by the methods developed by Beckmann-Rosenberg and Hosono-Sakurada. As the results of calculation by those methods, the absolute values of the coefficients of concentration dependence, |k_D|, were remarkably large; the values were 7.2, 16.3 and 22.4. The efficiency of Beckmann-Rosenberg's formula Dc=Do(1+k_DC) might be required to satisfy the condition where k_D is within one. It became clear that the coefficients of concentration dependence, k_<DBR> of acetylcholine chloride calculated by the method of Beckmann-Rosenberg corresponded to the initial concentration, although those of highly polymerised electrolytic compounds did not. Therefore, according to this experiment, Beckmann-Rosenberg's formula should be developed as the following generalized formula; Dc=D_o(AC√<C_o>+BC+1) and the concrete formula became Dc=D_o(225.49C√<C_o>-57.50C+1) where Dc was the diffusion coefficient on the concentration C which was calculated by Beckmann-Rosenberg's formula, Do was the diffusion coefficient of the limiting dilute concentration in the initial concentration Co and the symbol A and B are the experimental constant.

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