The Components of Acid-soluble Fraction Obtained from Rhizodrilus limasus (II) : Fractionation and Identification of the Components

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  • マミズイトミミズの酸可溶性化合物について(II) : 分画と同定
  • マミズイトミミズ ノ サン カヨウセイ カゴウブツ ニ ツイテ 2 ブンカク
  • 〓

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The acid soluble fraction obtained from a sludge worm, Rhizodrilus limasus, was subjected to an ion exchange chromatography using Dowex l, formate form. Some fractions separated by this procedure were moreover subjected to a rechromatography using the resin Cl form, and the fractions separated by the rechromatography were identified through the determination of acid-labile P, total P and pentose contents as well as their ultraviolet absorption curves. The obtained results were as follows. 1. Seven fractions were obtained by a chromatotraphy using Dowex l, formate form and the separation of these fractions in chromatogram was better than that in the previous paper. 2. Four fractions of these were identified as guanine, AMP, ADP, and ATP, respectively. 3. An attempt to culture Rhizodrilus limasus artificially was found to be unsuccessful.

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KJ00000066067

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