Acid Extraction and Column Chromatography of Pectic Substances from Laver, Porphyra tenera

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  • アサクサノリ・ペクチン質の酸性抽出とカラムクロマトグラフィについて
  • アサクサノリ ペクチンシツ ノ サンセイ チュウシュツ ト カラム クロマトグ
  • 〓

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In the previous paper, the authors tried to fractionate the pectic substances from laver, Porphyra tenera, into three fractions (water-soluble, sodium hexametaphosphate-soluble, and hydrochloric acid-soluble). But, in this paper, the extraction of the whole pectic substances in a lot was done under acidic condition (Scheme 1). It was found that the amount of the pectic substances prepared according to Scheme 1 was 2.18 mg (galacturonic acid eq.)/g fresh matter. The pectic substances from laver was, then, submitted to gel filtration on a column of Sephadex G-200. But, the filtration was not successful (Fig. 1). On the other hand, column chromatography on DEAE-cellulose (DE52) proved to be effective. It gave four peaks clearly (Fig. 2).

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KJ00000066171

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