センノサイドＡ、Ｂの免疫化学的測定法の開発について

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タイトル別名
  • Development of Immunochemical Method for Sennoside A and B
  • センノサイド A B ノ メンエキ カガクテキ ソクテイホウ ノ カイハツ ニ ツイテ

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説明

Sennoside A（SA）and sennoside B（SB）are the major purgative constituents of rhubarb and senna. Intestinal bacteria metabolize sennosides into rheinanthrone, which acts directly on the intestines as a purgative, functioning in the same way as natural prodrugs. Therefore, phytochemical studies of such plants require simple assays for the analysis of SA and SB, such as immunoassay systems that use monoclonal antibodies (MAbs) against bioactive compounds of low molecular weight. In fact, these methods are important tools for the qualitative and quantitative analysis of active constituents from natural medicines and plants. The production and characterization of anti-SA and anti-SB MAbs have been performed in our ongoing study of the production of MAbs against naturally occurring bioactive compounds. As an extension of antibodies generation, we report here the immunoassay systems as indicated below. 1. Establishment of an ELISA and immunochromatographic assays for SA and SB. 2. Development of eastern blotting technique for identification of SA and SB. 3. Immunohistochemical localization of SA in fresh rhubarb root.

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