電気的形質転換法を用いたcDNAライブラリーの作製

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  • デンキテキ ケイシツ テンカンホウ オ モチイタ c DNA ライブラリー ノ

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To transform Escherichia coli a plasmid DNA was introduced by electroporation. We improved transformation efficiency adding several chemicals, biological materials and/or preheating of the buffer solution which was added just affer electrodischarge. The improved method gave higher transformation efficiency comparing with that of Hanahan's. cDNA libraries for mRNAs appearing in the developing rice seeds and spinach seedlings were constructed using this method.

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