海洋細菌シュードモナスC55a-2株が産生する殺藻物質の精製

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  • Purification of an algicidal substance produced by marine Pseudomonas sp. C55a-2

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A marine bacterium, C55a-2 strain was selected among various algicidal bacteria isolated from coastal sea water of Kagoshima Bay,Japan, which killed diatom cells on a double layer agar plate. The algicidal strain was identified as Pseudomonas sp. tentatively onphysiological and biochemical examinations.Pseudomonas sp. C55a-2 was demonstrated to produce an algicidal substance extracellularly in marine nutrient medium Z-CII. Analgicidal substance was purified from the culture supernatant of strain C55a-2 by a series of purification steps including ethyl acetateextraction, Sep-Pak treatment, Sephadex LH-20 column chromatography and high performance liquid chromatography (HPLC) withMightysil RP-18GP column. The substance was shown to have algicidal activity against Chaetoceros ceratosporum, Chattonellamarina and Heterosigma akashiwo strains by use of a microplate culture method.

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