Tissue culture techniques for Gentiana spp. for seed production.

JASI Web Site

Bibliographic Information

Other Title
  • リンドウ種苗生産のための組織培養システム
  • リンドウ シュビョウ セイサン ノ タメ ノ ソシキ バイヨウ システム

Search this article

Abstract

The Gentian varieties cultivated by Iwate Prefecture are almost all F1 hybrids, so it requires a great deal of labor to maintain and multiply the parent stock and its selfed recessive traits. To that end, we tried to produce seeds using tissue culture methods, which can be more efficient and less laborious for parent stock maintenance and multiplication when compared to field cultivation. We tested leaf culture, overwintering bud disk culture, and liquid shaking culture techniques. Callus and shoot formation is possible through leaf cultures when explants are cultivated with a GC2 culture medium (a mixture of 0.5mg/l naphthalene acetic acid, 10.0mg/l thidiazuron, and 3% sucrose) added to a MS medium. We also examined family/genealogical line differences, individual differences, and the condition of the explant. Additionally, we took the overwintering buds formed through the cultivation process and cut a 5mm thick slice that included a bud node (called an overwintering bud disk). Callus and shoot formation occurred when cultivating the slice in the GC2 medium. We subcultured the seedlings that formed that overwintering buds in a hormone-free MS liquid medium, and a shaking culture performed at 120rpm encouraged growth. Furthermore, cultivating the shoots produced by the leaf culture at a 15deg C temperature promotes the growth and preservation of overwintering buds. By combining all of the above techniques, tissue culturing is seen to be practicable throughout the whole process flow, promoting growth, preservation of family lines, and root acclimation (except for a few family lines). Then we integrated all of these techniques together and devised a work schedule of actual seed production using these techniques, and determined the difficulty levels of the various family lines and tissue culture techniques. From the above research, we can expect that Gentian seed production using tissue culture techniques will contribute to a reliable supply of superior seeds and the promotion of efficient plant breeding, along with stable management for Gentian farmers and the development of Gentian production centers.

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top