Protective effects of free radical scavengers on transient ischemia-induced retinal ganglion cell death

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Objective: To evaluate the suppressive effect of two free radical scavengers, edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one) and Trolox® (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), on retinal ganglion cell death in transgenic Thy1-EGFP mice expressing enhanced green fluorescent protein in retinal ganglion cells. / Method: Retinal ischemia-reperfusion injury was performed on mice following intraperitoneal administration of edaravone or Trolox, and the number of retinal ganglion cells that survived ischemia/reperfusion was measured non-invasively over time using fluorescent fundus angiography. In addition, viable cells were counted in flat mount retinal specimens prepared from enucleated eyes. / Results: The fluorescence intensity at the fundus decreased steadily after transient ischemia/reperfusion. Administration of edaravone and Trolox suppressed this decrease by approximately 20% on day 7. These results were comparable with the reduction in the number of ganglion cells counted in flat mount retinal preparations. / Conclusion: Free radical scavengers effectively suppress ischemia-induced retinal ganglion cell death.


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