微量拡散法による炭酸脱水酵素の測定

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タイトル別名
  • ビリョウ カクサンホウ ニ ヨル タンサン ダッスイ コウソ ノ ソクテイ
  • A Microdiffusion Analytic Estimation of Carbonic Anhydrase

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We estimated the measurement of CO₂ for the determination of carbonic anhydrasa by the microdiffusion analysis. The absorbing time of the CO₂ by the Ba(OH)₂ is short in the little quantity of CO₂ (Fig. 2), thin stratum of CO₂ solution (Fig. 1), continual shake of unit (Fig. 1) and large quantity of Ba(OH)₂ (Fig. 2) but is constant through 0~37℃. (Eig. 3), and absorbing velccity is more rapid under the higher partial pressure of CO2 (Fig. 1 & 2). The measurement method of carbonic anhydrase by the microdiffusion analytic method is as accurate as manometric method (Fig. 4) and is simpler than the latter.

長崎大学水産学部研究報告, v.8, pp.270-274; 1959

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