カキ(Oyster)のシリアチン高含有リンタンパク質のアンジオテンシン-1 : 変換酵素阻害活性について

機関リポジトリ HANDLE Web Site

書誌事項

タイトル別名
  • カキ Oyster ノ シリアチン コウ ガンユウ リン タンパクシツ ノ ア
  • Angiotensin 1-Converting Enzyme Inhibiting Activity of Aminoethylphosphonic Acid-Containing Phosphoproteins of the Oyster, Crassostrea Gigas

この論文をさがす

説明

Author describe a method of extraction and partial purification of phospho-proteins from Oyster, Crassostrea gigas. The extraction of the phosphoproteins was carried out with hot water and with 0.02M NaHCO3 solution containing 5% NaCl. The Oyster, Crassostrea gigas contained two kinds of phosphoproteins in the hot water extracts (P-1, P-2) and three kinds of phosphoproteins in the alkaline soluble extracts (P-3, P-4 and P-5). The amino acid compositions of the phosphoproteins of the hot water extracts were characterized by relatively high percentage for glutamic acid and aspartic acid. The amino acid compositions of the each phosphoproteins of the alkaline soluble extracts were characterized by relatively high percentage for glutamic acid and threonine. The phosphono-compounds was found in the all phosphoproteins. It has been demonstrated that the peak 1, 2, 3, 4 and 5 contained the phosphonoproteins and that about 78%, 50%, 82%, 72% and 51% as phosphonate-phosphorus of total phosphorus. The inhibition of angiotensin 1 converting enzyme (ACE) by the five phosphoproteins was investigated in vitro. The IC50 values of these phosphoproteins (P-1～P-5) for ACE were 1.43, 0.27, 0.45, 0.33 and 0.17, respectively. The peak 5 had the most inhibition activity and showed 0.17 (mg protein / ml) inhibition against ACE at IC50 value.

収録刊行物

詳細情報 詳細情報について

問題の指摘

ページトップへ