Hemolymph acid-base balance of Akoya pearl oyster Pinctada fucata martensii with cannulated adductor muscle in normoxic conditions
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We collected the hemolymph anaerobically through a cannula inserted into the adductor muscle of Akoya pearl oyster Pinctada fucata martensii submerged in experimental seawater, and examined hemolymph pH, total CO_2 concentration (Tco_2), CO_2 partial pressure (Pco_2), and bicarbonate concentration ([HCO_3^–]) in order to evaluate the ability of the acid–base balance of P. fucata martensii in normoxic conditions. The mean values of hemolymph pH and Tco_2 were 7.544 and 2.21 mM/L, respectively. The apparent dissociation constant of carbonic acid (pKapp) was estimated using the following equation: pKapp = 225.712 – 97.096 • pH + 14.313 • pH^2 – 0.7035 • pH^3. Using mean values of pKapp (5.99585) and CO_2 solubility coefficient (αco_2, 37.02 µM/L/torr) determined in this study, the hemolymph Pco_2 and [HCO_3^–] were calculated as 1.69 torr and 2.14 mM/L, respectively. The non-bicarbonate buffer value (ꞵ_NB) was 0.967 slykes.
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- 水産大学校研究報告
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水産大学校研究報告 69 (1), 9-15, 2020-11
水産大学校
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詳細情報 詳細情報について
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- CRID
- 1050570000412177024
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- NII論文ID
- 120007089487
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- NII書誌ID
- AN00124678
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- ISSN
- 03709361
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- NDL書誌ID
- 031401120
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- 本文言語コード
- en
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- 資料種別
- departmental bulletin paper
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- データソース種別
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- IRDB
- NDL
- CiNii Articles