Conformational alterations in unidirectional ion transport of a light-driven chloride pump revealed using X-ray free electron lasers
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- 保坂, 俊彰
- RIKEN Center for Biosystems Dynamics Research
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- 野村, 高志
- RIKEN SPring-8 Center; Department of Life Science, Graduate School of Science, University of Hyogo
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- 久保, 稔
- Department of Life Science, Graduate School of Science, University of Hyogo
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- 中根, 崇智
- Department of Biological Sciences, Graduate School of Science, The University of Tokyo
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- ファンジア, ルオ
- RIKEN SPring-8 Center; Department of Cell Biology, Graduate School of Medicine, Kyoto University
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- 関根, 俊一
- RIKEN Center for Biosystems Dynamics Research
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- 伊藤, 拓宏
- RIKEN Center for Biosystems Dynamics Research
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- 村山, 和隆
- RIKEN Center for Biosystems Dynamics Research; Division of BiomedicalMeasurements and Diagnostics, Graduate School of Biomedical Engineering, Tohoku University
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- 伊原, 健太郎
- RIKEN Center for Biosystems Dynamics Research
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- 江原, 晴彦
- RIKEN Center for Biosystems Dynamics Research
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- 柏木, 一宏
- RIKEN Center for Biosystems Dynamics Research
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- 桂, 一茂
- RIKEN Center for Biosystems Dynamics Research
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- 赤坂, 領吾
- RIKEN Center for Biosystems Dynamics Research
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- 久野, 玉雄
- RIKEN Center for Biosystems Dynamics Research
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- 田中, 智之
- RIKEN SPring-8 Center; Department of Cell Biology, Graduate School of Medicine, Kyoto University
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- 田中, 里枝
- RIKEN SPring-8 Center; Department of Cell Biology, Graduate School of Medicine, Kyoto University
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- 有馬, 登志
- RIKEN SPring-8 Center; Department of Cell Biology, Graduate School of Medicine, Kyoto University
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- 山下, 鮎美
- RIKEN SPring-8 Center; Department of Cell Biology, Graduate School of Medicine, Kyoto University
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- 菅原, 道泰
- RIKEN SPring-8 Center
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- 内藤, 久志
- RIKEN SPring-8 Center
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- 松浦, 祥悟
- RIKEN SPring-8 Center
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- 吉澤, 晋
- Atmosphere and Ocean Research Institute, The University of Tokyo
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- 登野, 健介
- RIKEN SPring-8 Center; Japan Synchrotron Radiation Research Institute
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- 大和田, 成起
- RIKEN SPring-8 Center; Japan Synchrotron Radiation Research Institute
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- 濡木, 理
- Department of Biological Sciences, Graduate School of Science, The University of Tokyo
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- 染谷, 友美
- RIKEN Center for Biosystems Dynamics Research
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- 岩田, 想
- RIKEN SPring-8 Center; Department of Cell Biology, Graduate School of Medicine, Kyoto University
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- 南後, 恵理子
- RIKEN SPring-8 Center Institute of Multidisciplinary Research for Advanced Materials, Tohoku University
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- 白水, 美香子
- RIKEN Center for Biosystems Dynamics Research
説明
Light-driven chloride-pumping rhodopsins actively transport anions, including various halide ions, across cell membranes. Recent studies using time-resolved serial femtosecond crystallography (TR-SFX) have uncovered the structural changes and ion transfer mechanisms in light-driven cation-pumping rhodopsins. However, the mechanism by which the conformational changes pump an anion to achieve unidirectional ion transport, from the extracellular side to the cytoplasmic side, in anion-pumping rhodopsins remains enigmatic. We have collected TR-SFX data of Nonlabens marinus rhodopsin-3 (NM-R3), derived from a marine flavobacterium, at 10-µs and 1-ms time points after photoexcitation. Our structural analysis reveals the conformational alterations during ion transfer and after ion release. Movements of the retinal chromophore initially displace a conserved tryptophan to the cytoplasmic side of NM-R3, accompanied by a slight shift of the halide ion bound to the retinal. After ion release, the inward movements of helix C and helix G and the lateral displacements of the retinal block access to the extracellular side of NM-R3. Anomalous signal data have also been obtained from NM-R3 crystals containing iodide ions. The anomalous density maps provide insight into the halide binding site for ion transfer in NM-R3.
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 119 (9), 2022-03-01
Proceedings of the National Academy of Sciences
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詳細情報 詳細情報について
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- CRID
- 1050573243113573376
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- ISSN
- 10916490
- 00278424
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- HANDLE
- 2433/268241
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
- Crossref
- KAKEN