Watching a signaling protein function in real time via 100-ps time-resolved Laue crystallography
書誌事項
- 公開日
- 2012-11-20
- 資源種別
- journal article
- 権利情報
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- Copyright (c) 2012 National Academy of Sciences
- DOI
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- 10.1073/pnas.1210938109
- 公開者
- National Academy of Sciences
この論文をさがす
説明
To understand how signaling proteins function, it is crucial to know the time-ordered sequence of events that lead to the signaling state. We recently developed on the BioCARS 14-IDB beamline at the Advanced Photon Source the infrastructure required to characterize structural changes in protein crystals with near-atomic spatial resolution and 150-ps time resolution, and have used this capability to track the reversible photocycle of photoactive yellow protein (PYP) following trans-to-cis photoisomerization of its p-coumaric acid (pCA) chromophore over 10 decades of time. The first of four major intermediates characterized in this study is highly contorted, with the pCA carbonyl rotated nearly 90° out of the plane of the phenolate. A hydrogen bond between the pCA carbonyl and the Cys69 backbone constrains the chromophore in this unusual twisted conformation. Density functional theory calculations confirm that this structure is chemically plausible and corresponds to a strained cis intermediate. This unique structure is short-lived (?600 ps), has not been observed in prior cryocrystallography experiments, and is the progenitor of intermediates characterized in previous nanosecond time-resolved Laue crystallography studies. The structural transitions unveiled during the PYP photocycle include trans/cis isomerization, the breaking and making of hydrogen bonds, formation/relaxation of strain, and gated water penetration into the interior of the protein. This mechanistically detailed, near-atomic resolution description of the complete PYP photocycle provides a framework for understanding signal transduction in proteins, and for assessing and validating theoretical/computational approaches in protein biophysics.
収録刊行物
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- PNAS : proceedings of the National Academy of Sciences of the United States of America
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PNAS : proceedings of the National Academy of Sciences of the United States of America 109 (47), 19256-19261, 2012-11-20
National Academy of Sciences
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詳細情報 詳細情報について
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- CRID
- 1050577309352742016
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- NII論文ID
- 120005716810
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- NII書誌ID
- AA11726874
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- ISSN
- 10916490
- 00278424
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- HANDLE
- 10061/8581
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- PubMed
- 23132943
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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