The Caulobacter crescentus DciA promotes chromosome replication through topological loading of the DnaB replicative helicase at replication forks
-
- 尾﨑, 省吾
- 九州大学大学院薬学研究院臨床薬学部門分子生物薬学
-
- Wang, Dengyu
- 九州大学大学院薬学府
-
- 若杉, 泰敬
- 九州大学大学院薬学府
-
- 井谷, 直登
- 九州大学大学院薬学府
-
- 片山, 勉
- 九州大学大学院薬学研究院臨床薬学部門生命薬学
この論文をさがす
説明
The replicative DNA helicase translocates on single-stranded DNA to drive replication forks during chromosome replication. In most bacteria the ubiquitous replicative helicase, DnaB, co-evolved with the accessory subunit DciA, but how they function remains incompletely understood. Here, using the model bacterium Caulobacter crescentus, we demonstrate that DciA plays a prominent role in DNA replication fork maintenance. Cell cycle analyses using a synchronized Caulobacter cell population showed that cells devoid of DciA exhibit a severe delay in fork progression. Biochemical characterization revealed that the DnaB helicase in its default state forms a hexamer that inhibits self-loading onto single-stranded DNA. We found that upon binding to DciA, the DnaB hexamer undergoes conformational changes required for encircling single-stranded DNA, thereby establishing the replication fork. Further investigation of the functional structure of DciA revealed that the C-terminus of DciA includes conserved leucine residues responsible for DnaB binding and is essential for DciA in vivo functions. We propose that DciA stimulates loading of DnaB onto single strands through topological isomerization of the DnaB structure, thereby ensuring fork progression. Given that the DnaB-DciA modules are widespread among eubacterial species, our findings suggest that a common mechanism underlies chromosome replication.
収録刊行物
-
- Nucleic Acids Research (NAR)
-
Nucleic Acids Research (NAR) gkac1146-, 2022-12-09
Oxford University Press
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1050580007679984640
-
- NII書誌ID
- AA1203863X
-
- ISSN
- 13624962
- 03051048
-
- HANDLE
- 2324/6613535
-
- 本文言語コード
- en
-
- 資料種別
- journal article
-
- データソース種別
-
- IRDB