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Disaggregation Behavior of Amyloid β Fibrils by Anthocyanins Studied by Total-Internal-Reflection-Fluorescence Microscopy Coupled with a Wireless Quartz-Crystal Microbalance Biosensor
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Description
Noi K., Ikenaka K., Mochizuki H., et al. Disaggregation Behavior of Amyloid β Fibrils by Anthocyanins Studied by Total-Internal-Reflection-Fluorescence Microscopy Coupled with a Wireless Quartz-Crystal Microbalance Biosensor. Analytical Chemistry, 93(32), 11176-11183, August 17, 2021. Copyright © 2021, American Chemical Society. https://doi.org/10.1021/acs.analchem.1c01720.
Amyloid fibrils are formed from various proteins, some of which cause the corresponding neurodegenerative disorders, such as Alzheimer's and Parkinson's diseases. It has been reported that many compounds inhibit the formation of amyloid fibrils. Anthocyanins are flavonoid pigments present in fruits and vegetables, which are known to suppress symptoms related with Alzheimer's disease. However, the influence of anthocyanins on the amyloid fibril remains unclear. Here, we succeeded in the direct monitoring of the disaggregation reaction of single amyloid β (Aβ) fibrils by anthocyanins using total-internal-reflection-fluorescence microscopy with a quartz-crystal microbalance (TIRFM-QCM). It is found that the disassembly activity to the Aβ fibrils depends on the number of hydroxyl groups in six-membered ring B of anthocyanin, and only delphinidin-3-galactoside, possessing three hydroxyl groups there, shows high disassembly activity. Our results show the importance of the number of hydroxyl groups and demonstrate the usefulness of TIRFM-QCM as a powerful tool in studying interactions between amyloid fibrils and compounds.
Journal
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- Analytical Chemistry
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Analytical Chemistry 93 (32), 11176-11183, 2021-08-17
American Chemical Society
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Keywords
- Amyloid
- Amyloid beta-Peptides
- Chemical Sciences not elsewhere classified
- Cell Biology
- Biosensing Techniques
- Quartz
- TIRFM-QCM
- 540
- Biochemistry
- disassembly activity
- anthocyanin
- Peptide Fragments
- six-membered ring B
- Anthocyanins
- amyloid fibrils
- Microscopy, Fluorescence
- hydroxyl groups
- Amyloid β Fibrils
- Medicine
- Wireless Quartz-Crystal Microbalanc.
- Neuroscience
Details 詳細情報について
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- CRID
- 1050581168899374080
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- ISSN
- 15206882
- 00032700
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- HANDLE
- 11094/93315
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- PubMed
- 34351734
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- IRDB
- Crossref
- KAKEN
- OpenAIRE
