テンペイの抗酸化性有効物質の検索

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  • テンペイ ノ コウサンカセイ ユウコウ ブッシツ ノ ケンサク
  • Studies on the Antioxidative Factors in Tempeh

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For the purpose of finding new antioxidative factors in tempeh, fermented soybeans, investigation was made for detecting and concentrating the active new fraction, which protects oxidation of oil, from tempeh powder The following results were obtained : / 1) Hexane 2 : ethylalcohol 1 was most efficient among several solvents investigated to extract tempeh oil containing antioxidants. / 2) The antioxidative factors were concentrated in the emulsified fraction obtained after treating tempeh oil (extracted with hexane : alcohol) with 50% alcohol. / 3) The precipitate, which has the antioxidant activity, was obtained from about 80% acetone solution of the water layer extracted from the emulsified fraction after removing oil with petroleum ether. / 4) The active fraction was ethanol soluble and also water soluble. The fraction was positive for Emmerie-Engel Reaction. / 5) Fraction of Rf 0.04 and 0.17 with the paper partition chromatography (butanol 4 : acetic acid 1 : water 1) were both active to prevent the oxidation of oil. The UV maximum of the 0.04 and 0.17 fractions extracted with ethanol was 260 mμ.

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