Sperm preservation by freeze-drying for the conservation of wild animals.
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説明
Sperm preservation is a useful technique for the maintenance of biological resources in experimental and domestic animals, and in wild animals. A new preservation method has been developed that enables sperm to be stored for a long time in a refrigerator at 4°C. Sperm are freeze-dried in a solution containing 10 mM Tris and 1 mM EDTA. Using this method, liquid nitrogen is not required for the storage and transportation of sperm. We demonstrate that chimpanzee, giraffe, jaguar, weasel and the long-haired rat sperm remain viable after freeze-drying. In all species, pronuclei were formed after the injection of freeze-dried sperm into the mouse oocytes. Although preliminary, these results may be useful for the future establishment of "freeze-drying zoo" to conserve wild animals.
収録刊行物
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- PloS one
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PloS one 9 (11), e113381-, 2014-11-19
Public Library of Science
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詳細情報 詳細情報について
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- CRID
- 1050845760732492288
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- NII論文ID
- 120005522522
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- ISSN
- 19326203
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- HANDLE
- 2433/192770
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- PubMed
- 25409172
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
- Crossref
- CiNii Articles
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- OpenAIRE
