- 【Updated on May 12, 2025】 Integration of CiNii Dissertations and CiNii Books into CiNii Research
- Trial version of CiNii Research Knowledge Graph Search feature is available on CiNii Labs
- 【Updated on June 30, 2025】Suspension and deletion of data provided by Nikkei BP
- Regarding the recording of “Research Data” and “Evidence Data”
Establishment of a Therapeutic Anti-Pan HLA-Class II Monoclonal Antibody That Directly Induces Lymphoma Cell Death via Large Pore Formation.
-
- Francesco Bertolini
- editor
Search this article
Description
To develop a new therapeutic monoclonal Antibody (mAb) for Hodgkin lymphoma (HL), we immunized a BALB/c mouse with live HL cell lines, alternating between two HL cell lines. After hybridization, we screened the hybridoma clones by assessing direct cytotoxicity against a HL cell line not used for immunization. We developed this strategy for establishing mAb to reduce the risk of obtaining clonotypic mAb specific for single HL cell line. A newly established mouse anti-human mAb (4713) triggered cytoskeleton-dependent, but complement- and caspase-independent, cell death in HL cell lines, Burkitt lymphoma cell lines, and advanced adult T-cell leukemia cell lines. Intravenous injection of mAb 4713 in tumor-bearing SCID mice improved survival significantly. mAb 4713 was revealed to be a mouse anti-human pan-HLA class II mAb. Treatment with this mAb induced the formation of large pores on the surface of target lymphoma cells within 30 min. This finding suggests that the cell death process induced by this anti-pan HLA-class II mAb may involve the same death signals stimulated by a cytolytic anti-pan MHC class I mAb that also induces large pore formation. This multifaceted study supports the therapeutic potential of mAb 4713 for various forms of lymphoma.
Journal
-
- PLOS ONE
-
PLOS ONE 11 (3), e0150496-, 2016-03-30
Public Library of Science
- Tweet
Keywords
- Mice, Inbred BALB C
- Mice, Inbred ICR
- Cell Death
- Science
- Q
- R
- Histocompatibility Antigens Class II
- Antibodies, Monoclonal
- Antineoplastic Agents
- Mice, SCID
- Xenograft Model Antitumor Assays
- HEK293 Cells
- Cell Line, Tumor
- Medicine
- Animals
- Humans
- Cell Shape
- Cytoskeleton
- Research Article
- Signal Transduction
Details 詳細情報について
-
- CRID
- 1050845760768347776
-
- NII Article ID
- 120005838168
-
- ISSN
- 19326203
-
- HANDLE
- 2433/216532
-
- PubMed
- 27028595
-
- Text Lang
- en
-
- Article Type
- journal article
-
- Data Source
-
- IRDB
- Crossref
- CiNii Articles
- KAKEN
- OpenAIRE