Visualization of specific repetitive genomic sequences with fluorescent TALEs in Arabidopsis thaliana

DOI IR IR (HANDLE) HANDLE PDF View 1 Remaining Hide 12 Citations 54 References Open Access

Bibliographic Information

Other Title
  • Visualization of specific repetitive genomic sequences with fluorescent TALEs in<i>Arabidopsis thaliana</i>
  • TALE-mediated plant genome visualization

Search this article

Description

Live imaging of the dynamics of nuclear organization provides the opportunity to uncover the mechanisms responsible for four-dimensional genome architecture. Here, we describe the use of fluorescent protein (FP) fusions of transcription activator-like effectors (TALEs) to visualize endogenous genomic sequences in Arabidopsis thaliana. The ability to engineer sequence-specific TALEs permits the investigation of precise genomic sequences. We could detect TALE-FP signals associated with centromeric, telomeric, and rDNA repeats and the signal distribution was consistent with that observed by fluorescent in situ hybridization. TALE-FPs are advantageous because they permit the observation of intact tissues. We used our TALE-FP method to investigate the nuclei of several multicellular plant tissues including roots, hypocotyls, leaves, and flowers. Because TALE-FPs permit live-cell imaging, we successfully observed the temporal dynamics of centromeres and telomeres in plant organs. Fusing TALEs to multimeric FPs enhanced the signal intensity when observing telomeres. We found that the mobility of telomeres was different in subnuclear regions. Transgenic plants stably expressing TALE-FPs will provide new insights into chromatin organization and dynamics in multicellular organisms.

Journal

Citations (12)*help

See more

References(54)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top