Wide-range screening of anti-inflammatory compounds in tomato using LC-MS and elucidating the mechanism of their functions.
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- S, Mohri
- Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University
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- H, Takahashi
- Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University・KAGOME Tomato Discoveries Laboratory, Graduate School of Agriculture, Kyoto University
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- M, Sakai
- Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University
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- S, Takahashi
- Innovation Division, KAGOME CO., LTD.
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- N, Waki
- KAGOME Tomato Discoveries Laboratory, Graduate School of Agriculture, Kyoto University・ Innovation Division, KAGOME CO., LTD.
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- K, Aizawa
- Innovation Division, KAGOME CO., LTD.
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- H, Suganuma
- Innovation Division, KAGOME CO., LTD.
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- T, Ara
- KAGOME Tomato Discoveries Laboratory, Graduate School of Agriculture, Kyoto University
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- Y, Matsumura
- Laboratory of Quality Analysis and Assessment, Graduate School of Agriculture, Kyoto University
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- D, Shibata
- KAGOME Tomato Discoveries Laboratory, Graduate School of Agriculture, Kyoto University・Kazusa DNA Research Institutes, Kazusa-Kamatari
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- T, Goto
- Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University・Research Unit for Physiological Chemistry, Kyoto University
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- T, Kawada
- Laboratory of Molecular Function of Food, Graduate School of Agriculture, Kyoto University・Research Unit for Physiological Chemistry, Kyoto University
Abstract
Obesity-induced chronic inflammation is a key factor in type 2 diabetes. A vicious cycle involving pro-inflammatory mediators between adipocytes and macrophages is a common cause of chronic inflammation in the adipose tissue. Tomato is one of the most popular vegetables and is associated with a reduced risk of diabetes. However, the molecular mechanism underlying the effect of tomato on diabetes is unclear. In this study, we focused on anti-inflammatory compounds in tomato. We found that the extract of tomato reduced plasma glucose and inflammatory markers in mice. We screened anti-inflammatory fractions in tomato using lipopolysaccharide-stimulated RAW264.7 macrophages, and active compounds were estimated by liquid chromatography-mass spectrometry over a wide range. Surprisingly, a large number of compounds including oxylipin and coumarin derivatives were estimated as anti-inflammatory compounds. Especially, 9-oxo-octadecadienoic acid and daphnetin suppressed pro-inflammatory cytokines in RAW264.7 macrophages inhibiting mitogen-activated protein kinase phosphorylation and inhibitor of kappa B α protein degradation. These findings suggest that tomato containing diverse anti-inflammatory compounds ameliorates chronic inflammation in obese adipose tissue.
Journal
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- PloS one
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PloS one 13 (1), 2018-01-12
Public Library of Science (PLoS)
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Details 詳細情報について
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- CRID
- 1050845760787544448
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- NII Article ID
- 120006459972
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- ISSN
- 19326203
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- HANDLE
- 2433/230702
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- IRDB
- CiNii Articles