神経病原性レトロウイルスA8-Vのenv遺伝子発現に見られた奇妙なスプライシング
Bibliographic Information
- Other Title
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- シンケイ ビョウゲンセイ レトロウイルス A8 - V ノ env イデンシ ハツゲン ニ ミラレタ キミョウナ スプライシング
- shinkei byogensei retoroirusu A8 - V no env idenshi hatsugen ni mirareta kimyona supuraishingu
- Eccentric splicing found in env gene expression of neuropathogenic A8-V
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Abstract
type:text
Packaging cell lines PacNIH/A8β and PacNIH/A8δ, which were introduced by the DNA of expression vectors pA8(Ψ-)βand pA8(Ψ-)δ re spective1y, to produce pseud-retroviral particles in the retroviral vector system, were established. Both the expression vectors were constructed using the genes of A8-V, which is an isolate from Friend murine leukemia virus(F-MLV) and shows higher proliferation rates in the central nervous system(CNS) of rats and mice compared with original F-MLV. These two expression vectors utilize a different polyadenylation signal (polyA signal) for the expression of retroviral genes. The former employs a 0.85kb fragment in the SV40 early region as a polyA signal, and the latter, a 0.13kb of the same region. PacNIH/A8β showed an unstable expression of the env gene in the A8-V genome, whereas all of the retroviral gene expressions in the PacNIH/A8δ were perfect. An occurrence of abnormal splicing in the mRNA for the env gene in the PacNIH/A8β cells was suggested.
原著
Journal
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- 慶應医学
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慶應医学 83 (1), 29-35, 2006-03
慶應医学会
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Details 詳細情報について
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- CRID
- 1050845762329431168
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- NII Article ID
- 10017544056
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- NII Book ID
- AN00069296
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- ISSN
- 03685179
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- NDL BIB ID
- 7899798
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- Text Lang
- ja
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- Article Type
- journal article
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- Data Source
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- IRDB
- NDL
- CiNii Articles