抗原特異的B細胞を用いた抗原提示に関する基礎的研究

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  • ANALYSIS OF ANTIGEN PRESENTATION TO ANTIGEN SPECIFIC T CELLS USING B LYMPHOMA CELLS SPECIFIC FOR DEFINED ANTIGEN

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type:Article

We have studied the function of antigen specific immunoglobulin (Ig) receptors in antigen presentation. Monoclonal B cell lines expressing antigen specific surface IgM, IgD or IgG were established by transferring the gene encoding heavy (μ, δ or γ) and light (κ) chains specific for the　hapten 2, 4, 6-trinitropheny (TNP) into Ⅰa bearing B cell lymphoma A 20-2 J and WEHI 279 lines. Transfectants were compared in a quantitative assay for the ability to present TNP-carrier to carrier-specific T helper (Th) cells. The A 20-2 J-derived transfectants expressing μκтɴр, δκтɴр or γκтɴр had similiar amounts of the TNP-specific idiotype on their surface and showed an equal capacity to present antigen, which was about 1000-fold more efficient than that of the parental line. However, the γκтɴр transfectant of WEHI 279 line expressed lower amounts of the idiotype than μκтɴр or δκтɴр transfectant and exhibited a decreased capacity of antigen presentation. It was suggested that the antigen presenting capacity of antigen-specific B cells depends on the amount of the idiotype expressed, regardless of its Ig isotype. In addition, a Th hybridoma line used in this study exhibited cytotoxic activity to antigen presenting cell as well as IL-2 production when it was activated. Interestingly, cytotoxicities were also found to NK-resistant cells and to human tumor cells. The target recognition of Th cells was supposed to be different from that of NK cells or cytotoxic T cells.

identifier:奈良医学雑誌 Vol.44 No.3 p.137-145

identifier:04695550

identifier:13450069

identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/1800

identifier:奈良医学雑誌, 44(4): 137-145

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