Studies on the Binding of Xenopus Transcription Factor(TF3A)to the 5S RNA Gene by Scanning Transmission Electron Microscopy

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  • Studies on the Binding of Xenopus Transcription Factor (TFIIIA) to the 5S RNA Gene by Scanning Transmission Electron Microscopy
  • Studies on the Binding of Xenopus Trans
  • Studies on the Binding of Xenopus Transcription Factor (TFIIIA) to the 5S RNA Gene by Scanning Transmission Electron Microscopy

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Complexes of the Xenopus laevis somatic 5S gene-specific transcription factor, TFIIIA, and cloned somatic 5S DNA have been examined by scanning transmission electron microscopy (STEM). DNA contour length implies that the length of the DNA is compatible with form B DNA. Mass measurement of TFIIIA specifically bound to DNA as determined by the STEM, Mr=40,000 ± 4000 is in agreement with previous values obtained by gel migration. Preliminary measurement of the binding of TFIIIA to the 5S gene suggests that TFIIIA prefers to bind to the 3' half of the internal control region.

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