Ultrastructural Analysis of Unfertilized Abalone Egg Cortex using Thin Section and Quick-Freeze Deep-Etch Rotary-Shadow Electron Microscopy

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Ultrastructure of the egg cortex isolated from unfertilized abalone eggs was studied by thin section and quick-freeze, deep-etch, rotary-shadow electron microscopy. The isolation procedure employed in the present study yielded large segments of the egg plasma membrane. Thin sections of isolated egg cortex revealed the existence of fine filamentous structures closely surrounding the egg microvilli protruding from the egg surface. The presence of thin filaments extending within the microvilli was also evident in thin sections. Furthermore, the quick-freeze deep-etch rotary-shadow technique which minimizes the detrimental effects of chemical fixation provided three-dimensional view of the ultrastructure of the egg surface. In quick-freeze deep-etch images of isolated egg cortices, dense networks of filaments interconnecting the neighboring microvilli throughout the external surface of the egg were identified. The dense networks of filaments anchored on the egg surface may participate in ensuring the sperm-egg interactions for efficient fertilization.

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