Estimation of CO2 partial pressure and bicarbonate concentration in the hemolymph of the noble scallop Mimachlamys nobilis

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We examined hemolymph pH, total CO2 content (Tco2, mM/l), CO2 partial pressure (Pco2, mmHg) and bicarbonate concentration ([HCO3- ], mM/l) in order to evaluate the acid-base balance of the noble scallop Mimachlamys nobilis in normoxic conditions. Hemolymph was collected anaerobically through a cannula after catheterization of the adductor muscle. The mean values of hemolymph pH and Tco2 were 7.442 and 1.50 mM/l, respectively. Using the CO2 solubility coefficient and apparent dissociation constant of carbonic acid determined in this study, Pco2 and [HCO3- ] were calculated as 1.55 mmHg and 1.44 mM/l, respectively. These values were in same range as those of the hemolymph of other marine bivalves of Mytiloida, Pterioida and Ostreoida.

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