- 【Updated on May 12, 2025】 Integration of CiNii Dissertations and CiNii Books into CiNii Research
- Trial version of CiNii Research Knowledge Graph Search feature is available on CiNii Labs
- 【Updated on June 30, 2025】Suspension and deletion of data provided by Nikkei BP
- Regarding the recording of “Research Data” and “Evidence Data”
5′-Terminal stem-loop of carnation ringspot virus RNA1 is required for the efficient amplification of viral RNAs
-
- Kaido, Masanori
- Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
-
- Nagano, Hideaki
- Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
-
- Omote, Kazunori
- Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
-
- Takano, Yoshitaka
- Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
-
- Mise, Kazuyuki
- Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
-
- Okuno, Tetsuro
- Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University
Search this article
Description
Carnation ringspot virus (CRSV) is the prototype virus of the genus Dianthovirus. Full-length cDNAs of CRSV strainsPV-0097 and PV-21 were constructed and the infectivity of in vitro transcripts was analyzed. Infectivity of PV-0097 and PV-21 to several plants was markedly higher than that of 1.30, a previously reported infectious CRSV clone. Overall RNA sequences of these viruses were similar, but PV-0097 and PV-21 contained additional nucleotides at the 5′ end of RNA1. Stem-loop structures were predicted in the 5′-terminal region of PV-0097 and PV-21 RNA1 but not in 1.30 RNA1. Mutant CRSV 1.30 RNA1 that contains the terminal 4 nucleotides of PV-0097, predicted to fold a 5′-terminal stem-loop structure, recovered higher level accumulation of viral RNAs in the inoculated protoplasts and leaves of Nicotiana benthamiana. These results suggest that the 5′-terminal stem-loop structure of CRSV RNA1 plays an important role in efficient amplification of the virus.
Journal
-
- Virus Research
-
Virus Research 265 138-142, 2019-05
Elsevier B.V.
- Tweet
Details 詳細情報について
-
- CRID
- 1050845763174846592
-
- NII Article ID
- 120006621537
-
- ISSN
- 01681702
- 18727492
-
- HANDLE
- 2433/240991
-
- PubMed
- 30890436
-
- Text Lang
- en
-
- Article Type
- journal article
-
- Data Source
-
- IRDB
- Crossref
- CiNii Articles
- KAKEN
- OpenAIRE