β-1.4-マンノビオースを用いたβ-マンノシダーゼ活性測定法

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  • Measurement of β-Mannosidase Activity Using β-1,4-Mannobiose
  • ベータ 1 4 マンノビオース オ モチイタ ベータ マンノシダーゼ カッセイ

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"Glucose C II - Test Wako" (Glucose Oxidase (GOD) Reagent) reacts glucose well, and is sensitive against xylose and mannose (M_1). Using this reagent and β-1, 4-mannobiose (M_2), Aspergillus niger β-mannosidase activily could be measured. The enzyme hydrolyzed M_2 to produce M_1, and no transfer product was detected in the thin-layer chromatography. Optimal pH of the enzyme using M_2 was measured to be the same around pH 3-4 as using p-nitrophenyl β-D-mannopyranoside (PNPM). The enzyme activity measured by the developed method seemed to be identical with the activity using PNPM as substrate. This result indicate that β-mannosidase activity can be measured by the developed method using M_2 instead of PNPM.

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