Separation and Detection of Serum Lipoproteins by Electrophoresis on Cellulose Acetate Membrane

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  • セルロースアセテート膜電気泳動法による血清リポタンパク質の分析条件の検討
  • セルロースアセテートマク デンキ エイドウホウ ニ ヨル ケッセイ リポタンパクシツ ノ ブンセキ ジョウケン ノ ケントウ

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Electrophoresis using cellulose acetate membranes as support media is widely used for clinical examination of serum protein. In this study, analytical conditions of serum lipoprotein with cellulose acetate electrophoresis were investigated. Similar to the analysis of serum protein by cellulose acetate electrophoresis, a good separation and stained image was obtained by cellulose acetate electrophoresis using sodium barbital buffer solution and staining with Sudan Red 7B. Based on the measurement of the density of the bands of α- and β-lipoprotein fractions including HDL and LDL, respectively, the α- and β-lipoprotein fraction ratio（α/β ratio）was calculated. This value was found to be close to the HDLcholesterol / LDL-cholesterol value, which is attracting attention as a clinical test value related to a risk factor for sudden cardiac death. Because analysis of serum lipoprotein by cellulose acetate electrophoresis established in the report can be performed with as little as 2μL of human serum sample and the whole procedure can be completed within 2 hours, it is considered to be a valuable program for an experimental course in medical education.

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