Effect of calcium ion on the thermal denaturation of subfragment-1 and rod regions of squid myosin upon the heating of myofibrils
Bibliographic Information
- Other Title
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- スルメイカ筋原線維の加熱によるミオシン・ サブフラグメント-1およびロッド変性に対するCa2+の影響
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Abstract
Thermal inactivation of Ca^[2+]-ATPase of squid myofibrils was significantly suppressed in the presence of Ca^[2+]. Monomeric myosin content decreased much faster than Ca^[2+]-ATPase inactivation in Ca-medium, which was well explained by the fast rod denaturation. In contrast, rod denaturation was slower than S-1 in EDTA medium. Decrease in monomeric myosin content was explained by faster S-1 denaturation. Comparing the S-1 and rod denaturation rates at a fixed temperature, it was concluded that S-1 denaturation was suppressed by Ca^[2+] whereas the rod portion was not. Unfolding experiment with isolated myosin rod confirmed no stabilizing effect of Ca2+ on rod. It was concluded that significant stabilization of S-1 portion by Ca2+ generated apparently different myosin denaturation pattern in two media.
Journal
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- Fisheries Science
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Fisheries Science 78 (1), 163-168, 2012-01
Springer Japan
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Keywords
Details 詳細情報について
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- CRID
- 1050845763939800448
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- NII Article ID
- 10030025383
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- NII Book ID
- AA10993718
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- HANDLE
- 2115/48256
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- NDL BIB ID
- 023342632
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- ISSN
- 09199268
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- IRDB
- NDL
- CiNii Articles
