Intracellular stability of 2′-OMe-4′-thioribonucleoside modified siRNA leads to long-term RNAi effect
書誌事項
- タイトル別名
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- Intracellular stability of 2'-OMe-4'-thioribonucleoside modified siRNA leads to long-term RNAi effect
- Intracellular stability of 2'-OMe-4'-thioribonucleoside modified siRNA leads to ling-term RNAi effect
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説明
Chemically modified siRNAs are expected to have resistance toward nuclease degradation and good thermal stability in duplex formation for in vivo applications. We have recently found that 2'-OMe-4'-thioRNA, a hybrid chemical modification based on 2'-OMeRNA and 4'-thioRNA, has high hybridization affinity for complementary RNA and significant resistance toward degradation in human plasma. These results prompted us to develop chemically modified siRNAs using 2'-OMe-4'-thioribonucleosides for therapeutic application. Effective modification patterns were screened with a luciferase reporter assay. The best modification pattern of siRNA, which conferred duration of the genesilencing effect without loss of RNAi activity, was identified. Quantification of the remaining siRNA in HeLa-luc cells using a Heat-in-Triton (HIT) qRT-PCR revealed that the intracellular stability of the siRNA modified with 2'-OMe-4'-thioribonucleosides contributed significantly to the duration of its RNAi activity.
収録刊行物
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- Nucleic Acids Research
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Nucleic Acids Research 40 (12), 5787-5793, 2012-07
Oxford University Press
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詳細情報 詳細情報について
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- CRID
- 1050845763940987392
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- NII論文ID
- 120004405542
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- ISSN
- 13624962
- 03051048
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- HANDLE
- 2115/49693
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- PubMed
- 22411910
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- Crossref
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