Cytoplasmic control of Rab family small <scp>GTP</scp> ases through <scp>BAG</scp> 6
書誌事項
- タイトル別名
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- Cytoplasmic control of Rab family small GTPases through BAG6
この論文をさがす
説明
Rab family small GTPases are master regulators of distinct steps of intracellular vesicle trafficking in eukaryotic cells. GDP-bound cytoplasmic forms of Rab proteins are prone to aggregation due to the exposure of hydrophobic groups but the machinery that determines the fate of Rab species in the cytosol has not been elucidated in detail. In this study, we find that BAG6 (BAT3/Scythe) predominantly recognizes a cryptic portion of GDP-associated Rab8a, while its major GTP-bound active form is not recognized. The hydrophobic residues of the Switch I region of Rab8a are essential for its interaction with BAG6 and the degradation of GDP-Rab8a via the ubiquitin-proteasome system. BAG6 prevents the excess accumulation of inactive Rab8a, whose accumulation impairs intracellular membrane trafficking. BAG6 binds not only Rab8a but also a functionally distinct set of Rab family proteins, and is also required for the correct distribution of Golgi and endosomal markers. From these observations, we suggest that Rab proteins represent a novel set of substrates for BAG6, and the BAG6-mediated pathway is associated with the regulation of membrane vesicle trafficking events in mammalian cells.
収録刊行物
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- EMBO reports
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EMBO reports 20 (4), e46794-, 2019-02
EMBO press
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キーワード
- Cytoplasm
- Golgi Apparatus
- Endosomes
- Endoplasmic Reticulum
- Models, Biological
- Article
- Cell Line
- Cytosol
- Animals
- Humans
- Protein Interaction Domains and Motifs
- Amino Acid Sequence
- RNA, Small Interfering
- Membrane & Intracellular Transport
- Ubiquitin
- membrane trafficking
- Cell Membrane
- BAG6
- ubiquitin‐proteasome system
- Post-translational Modifications, Proteolysis & Proteomics
- Articles
- Rab8a
- Protein Transport
- rab GTP-Binding Proteins
- Proteolysis
- Gene Deletion
- Molecular Chaperones
- Protein Binding
- Rab GTP‐binding proteins
詳細情報 詳細情報について
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- CRID
- 1050845764128147200
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- NII論文ID
- 120007127921
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- NII書誌ID
- AA11472481
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- ISSN
- 14693178
- 1469221X
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- HANDLE
- 2241/00157181
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- PubMed
- 30804014
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
- Crossref
- CiNii Articles
- KAKEN
- OpenAIRE