蛍光酵素抗体法を用いたシラカバ及びハンノキ花粉に対する血漿IgE抗体の測定

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  • ケイコウ コウソ コウタイホウ オ モチイタ シラカバ オヨビ ハンノキ カフン ニ タイスル ケッショウ IgE コウタイ ノ ソクテイ
  • Measurement of Plasma IgE Antibodies against Birch and Alder Pollen Allergens by Fluorescence Enzyme-immunosorbent Assays

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We have established the fluorescence enzyme-immunosorbent assay (F-ELISA) for detecting plasma antibody immunoglobulin E(IgE) against birch pollen. The plasma samples were collected from volunteers containing individuals with symptoms of pollinosis, and measured by the F-ELISA and CAP system, which is widely used in clinical test companies. The results obtained by the F-ELISA exactly corresponded with those obtained by CAP system (γ2＝0.987, η＝13), indicating that our F-ELISA method might be very useful for detecting the anti-birch pollen IgE titers. Furthermore, we have also established the F-ELISA for detecting antibody IgE against alder pollen, which has been reported to have common antigens included in birch pollen. And then we measured the IgE antibodies against birch and alder pollen in plasma from 18 volunteers using two F-ELISAs, respectively. As a result, the levels of both antibody titers in individuals with symptoms of pollinosis were found to be higher than those in healthy individuals. In addition, the levels of anti-birch pollen IgE titer were correlated with the levels of anti-alder pollen IgE titer (r2=0.773, n-18). This finding supports the concept that antigens in birch and alder pollens share allergenic epitopes, and suggests that the patients with birch pollinosis need to take care during not only scattering period of birch pollen, but also that of alder pollen. Because these F-ELISAs are simple in procedure and not expensive compared with CAP system, it is suggested that they might be available for surveillance of anti-pollen IgE antibody at a large scale.

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