A novel motif of Rad51 serves as an interaction hub for recombination auxiliary factors.
書誌事項
- タイトル別名
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- A novel motif of Rad51 serves as an interaction hub for recombination auxiliary factors
抄録
Homologous recombination (HR) is essential for maintaining genome stability. Although Rad51 is the key protein that drives HR, multiple auxiliary factors interact with Rad51 to potentiate its activity. Here, we present an interdisciplinary characterization of the interactions between Rad51 and these factors. Through structural analysis, we identified an evolutionarily conserved acidic patch of Rad51. The neutralization of this patch completely abolished recombinational DNA repair due to defects in the recruitment of Rad51 to DNA damage sites. This acidic patch was found to be important for the interaction with Rad55-Rad57 and essential for the interaction with Rad52. Furthermore, biochemical reconstitutions demonstrated that neutralization of this acidic patch also impaired the interaction with Rad54, indicating that a single motif is important for the interaction with multiple auxiliary factors. We propose that this patch is a fundamental motif that facilitates interactions with auxiliary factors and is therefore essential for recombinational DNA repair.
identifier:oai:t2r2.star.titech.ac.jp:50586839
収録刊行物
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- eLife
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eLife 10 e64131-, 2021-01
eLife Sciences Publications
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詳細情報 詳細情報について
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- CRID
- 1050851862098346880
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- NII論文ID
- 120007124436
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- ISSN
- 2050084X
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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