Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA
説明
X-ray free-electron lasers (XFELs) have opened new opportunities for timeresolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TRSFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented.
収録刊行物
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- Journal of Synchrotron Radiation
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Journal of Synchrotron Radiation 24 (5), 1086-1091, 2017-09-01
International Union of Crystallography
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キーワード
- serial femtosecond crystallography
- PHOTOACTIVE YELLOW PROTEIN
- BACTERIORHODOPSIN
- XFEL
- time-resolved X-ray crystallography
- Atom and Molecular Physics and Optics
- Short Communications
- 535
- pump and probe
- FREE-ELECTRON LASER
- X-RAY-DIFFRACTION
- PROTEIN-STRUCTURE DETERMINATION
- Atom- och molekylfysik och optik
- STRUCTURAL-CHANGES
詳細情報 詳細情報について
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- CRID
- 1050856995323810560
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- NII論文ID
- 120006363292
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- ISSN
- 16005775
- 09090495
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- HANDLE
- 20.500.14094/90004340
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- PubMed
- 28862633
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
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