Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA

Kubo, Minoru, Nango, Eriko, Tono, Kensuke, 木村, 哲就, Owada, Shigeki, Song, Changyong, Mafune, Fumitaka, Miyajima, Ken, Takeda, Yoshihiro, Kohno, Jun-ya, Miyauchi, Naoya, Nakane, Takanori, Tanaka, Tomoyuki, Nomura, Takashi, Davidsson, Jan, Tanaka, Rie, Murata, Michio, Kameshima, Takashi, Hatsui, Takaki, Joti, Yasumasa, Neutze, Richard, Yabashi, Makina, Iwata, So

X-ray free-electron lasers (XFELs) have opened new opportunities for timeresolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TRSFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented.

Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA

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収録刊行物

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Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA

説明

収録刊行物

詳細情報 詳細情報について

書き出し

問題の指摘

詳細情報詳細情報について