Erythropoietin receptor signal is crucial for periodontal ligament stem cell-based tissue reconstruction in periodontal disease
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- Zakaria, MHD. Fouad
- Department of Molecular Cell Biology and Oral Anatomy, Graduate School of Dental Science, Kyushu University Department of Periodontology, Graduate School of Dental Science, Kyushu University
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- Sonoda, Soichiro
- Department of Molecular Cell Biology and Oral Anatomy, Graduate School of Dental Science, Kyushu University
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- Kato, Hiroki
- Department of Molecular Cell Biology and Oral Anatomy, Graduate School of Dental Science, Kyushu University
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- Ma, Lan
- Department of Molecular Cell Biology and Oral Anatomy, Graduate School of Dental Science, Kyushu University Guangdong Provincial Key Laboratory of Stomatology,South China Center of Craniofacial Stem Cell Research, Hospital of Stomatology, Guanghua School ofStomatology, Sun Yat-sen University
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- Uehara, Norihisa
- Department of Molecular Cell Biology and Oral Anatomy, Graduate School of Dental Science, Kyushu University
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- Kyumoto, Yukari
- Department of Molecular Cell Biology and Oral Anatomy, Graduate School of Dental Science, Kyushu University
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- Sharifa, M. Majd
- Department of Molecular Cell Biology and Oral Anatomy, Graduate School of Dental Science, Kyushu University
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- Yu, Liting
- Department of Molecular Cell Biology and Oral Anatomy, Graduate School of Dental Science, Kyushu University
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- Dai, Lisha
- Department of Molecular Cell Biology and Oral Anatomy, Graduate School of Dental Science, Kyushu University
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- Yamauchi, Erika
- Department of Oral and Maxillofacial Radiology, Graduate School of Dental Science, Kyushu University
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- Aijima, Reona
- Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Saga University
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- Yamaza, Haruyoshi
- Department of Pediatric Dentistry, Graduate School of Dental Science, Kyushu University
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- Nishimura, Fusanori
- Department of Periodontology, Graduate School of Dental Science, Kyushu University
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- Yamaza, Takayoshi
- Department of Molecular Cell Biology and Oral Anatomy, Graduate School of Dental Science, Kyushu University
Description
Alveolar bone loss caused by periodontal disease eventually leads to tooth loss. Periodontal ligament stem cells (PDLSCs) are the tissue-specific cells for maintaining and repairing the periodontal ligament, cementum, and alveolar bone. Here, we investigated the role of erythropoietin receptor (EPOR), which regulates the microenvironment-modulating function of mesenchymal stem cells, in PDLSC-based periodontal therapy. We isolated PDLSCs from patients with chronic periodontal disease and healthy donors, referred to as PD-PDLSCs and Cont-PDLSCs, respectively. PD-PDLSCs exhibited reduced potency of periodontal tissue regeneration and lower expression of EPOR compared to Cont-PDLSCs. EPOR-silencing suppressed the potency of Cont-PDLSCs mimicking PD-PDLSCs, whereas EPO-mediated EPOR activation rejuvenated the reduced potency of PD-PDLSCs. Furthermore, we locally transplanted EPOR-silenced and EPOR-activated PDLSCs into the gingiva around the teeth of ligament-induced periodontitis model mice and demonstrated that EPOR in PDLSCs participated in the regeneration of the periodontal ligament, cementum, and alveolar bone in the ligated teeth. The EPOR-mediated paracrine function of PDLSCs maintains periodontal immune suppression and bone metabolic balance via osteoclasts and osteoblasts in the periodontitis model mice. Taken together, these results suggest that EPOR signaling is crucial for PDLSC-based periodontal regeneration and paves the way for the development of novel options for periodontal therapy.
Journal
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- Scientific Reports
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Scientific Reports 14 6719-, 2024-03-20
Springer
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Details 詳細情報について
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- CRID
- 1050863241233759232
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- ISSN
- 20452322
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- HANDLE
- 2324/7178660
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- IRDB