Non-specific cytotoxic cell receptor protein-1 (NCCRP-1) is involved in anti-parasite innate CD8^+ T cell-mediated cytotoxicity in ginbuna crucian carp

DOI 機関リポジトリ (HANDLE) オープンアクセス
  • 助田, 将樹
    九州大学大学院生物資源環境科学府生命機能科学専攻海洋生命化学講座
  • Prakash, Harsha
    九州大学大学院生物資源環境科学府生命機能科学専攻海洋生命化学講座
  • 長澤, 貴宏
    九州大学大学院生物資源環境科学府生命機能科学専攻海洋生命化学講座
  • 中尾, 実樹
    九州大学大学院生物資源環境科学府生命機能科学専攻
  • 杣本, 智軌
    九州大学大学院生物資源環境科学府生命機能科学専攻

この論文をさがす

説明

CD8^+ cytotoxic T cells (CTLs) are a main cellular component of adaptive immunity. Our previous research has shown that CD8^+ cells demonstrate spontaneous cytotoxic activity against the parasite Ichthyophthirius multifiliis in ginbuna crucian carp, suggesting that CD8^+ cells play an important role in innate immunity. Herein, we investigated the molecules and cellular signal pathways involved in the cytotoxic response of ginbuna crucian carp. We considered non-specific cytotoxic receptor protein-1 (NCCRP-1) as candidate molecule for parasite recognition. We detected NCCRP-1 protein in CD8^+ cells and the thymus as well as in other cells and tissues. CD8^+ cells expressed mRNA for NCCRP-1, Jak2, and T cell-related molecules. In addition, treatment with a peptide containing the presumed antigen recognition site of ginbuna NCCRP-1 significantly inhibited the cytotoxic activity of CD8^+ cells against the parasites. The cytotoxic activity of CD8^+ cells was significantly inhibited by treatment with the JAK1/2 inhibitor baricitinib. These results suggest that teleost CTLs recognize I. multifiliis through NCCRP-1 and are activated by JAK/STAT signaling.

収録刊行物

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ