細菌汚染された粗面のチタン表面に対する除染法と生物学的成長因子を用いた骨分化の検討

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  • Effects of chemomechanical decontamination methods and biological growth factors for bone differentiation on rough titanium surfaces
  • サイキン オセン サレタ ソメン ノ チタン ヒョウメン ニ タイスル ジョセンホウ ト セイブツガクテキ セイチョウ インシ オ モチイタ ホネ ブンカ ノ ケントウ

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Peri−implantitis is a plaque−associated pathological condition affecting both the soft and hard tissues around implants. Ichioka et al. (2019) indicated that air−abrasive debridement followed by alkaline electrolyzed water (AEW) beneficial in restoring cytocompatibility of previously bacteria−contaminated smooth titanium surfaces. Since currently available implants are made of rough surfaces, it is also interesting to evaluate the potential effects of the proposed method on the rough surface. In addition, the present in vitro study assessed the effects of biological growth factors on the osteogenic cells when cultivated on the decontaminated titanium rough surfaces. Contaminated titanium discs were mechanically cleaned using air−abrasive debridement with erythritol powder. The discs were then immersed either in 0.9% NaCl, 0.05% AEW, or 3% H2O2 for 1 minute. X−ray photoelectron spectroscopy (XPS) was used to evaluate the chemical surface properties of the treated titanium discs. In addition, osteoblast−like cells (MC3T3−E1 ) were cultured on the treated titanium discs in cell medium in adjunct to either enamel matrix derivative (EMD) or basic fibroblast growth factor (FGF−2) . The cytocompatibility was evaluated by counting the number of attached cells and measuring cell spread area. The bone differentiation was evaluated by ALP expression of the cells. XPS analysis demonstrated that AEW group showed significantly lower organic contamination than the NaCl and H2O2 groups. The number of attached cells and their spread in AEW group were significantly greater compared to those in the other groups. ALP expression was also significantly enhanced in the AEW group compared with that in the NaCl group. No additional effects of EMD or FGF−2 in ALP expression were observed. Air−abrasive debridement followed by AEW may be beneficial to restore cytocompatibility and enhance bone differentiation of previously bacterial contaminated rough titanium surfaces. Our findings suggest that neither FGF−2 nor EMD provide additional benefits in the differentiation of MC3T3−E1 cells.

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