Brain Pericytes are a Major Source of Lipocalin-type Prostaglandin D2 Synthase in the Cerebral Cortex after Ischemic Stroke

Lipocalin-type prostaglandin D2 Synthase (LPGDS), a major cerebrospinal fluid (CSF) protein known as β-trace, reportedly scavenges biliverdin in the CSF of patients with subarachnoid hemorrhage and functions as a major amyloid β-chaperone, suggesting a clearance role in the central nervous system. However, the source of LPGDS in the cerebral cortex following stroke has not been elucidated. We examined changes in the cellular localization of LPGDS in post-stroke cerebral cortices of mice. Immunofluorescence staining were performed to determine the localization of LPGDS. Immunoelectron microscopy for LPGDS was also performed. In normal cerebral cortices, immunoreactive LPGDS structures were rarely observed. However, higher LPGDS expression levels were observed on PDGFRβ+ pericytes and nestin+ stem cells. Immunoelectron microscopy confirmed that pericytes in association with the endothelial cells expressed LPGDS. It is strongly expressed in brain pericytes following ischemic insult, suggesting that the ischemic pericytes are the major site of LPGDS expression following stroke. Knowledge of these expression profiles may be useful for future PC-based stem cell therapies.

2019年度

収集根拠 : 博士論文（自動収集）
資料形態 : テキストデータ
コレクション : 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
Lipocalin-type prostaglandin D2 Synthase (LPGDS), a major cerebrospinal fluid (CSF) protein known as β-trace, reportedly scavenges biliverdin in the CSF of patients with subarachnoid hemorrhage and functions as a major amyloid β-chaperone, suggesting a clearance role in the central nervous system. However, the source of LPGDS in the cerebral cortex following stroke has not been elucidated. We examined changes in the cellular localization of LPGDS in post-stroke cerebral cortices of mice. Immunofluorescence staining were performed to determine the localization of LPGDS. Immunoelectron microscopy for LPGDS was also performed. In normal cerebral cortices, immunoreactive LPGDS structures were rarely observed. However, higher LPGDS expression levels were observed on PDGFRβ+ pericytes and nestin+ stem cells. Immunoelectron microscopy confirmed that pericytes in association with the endothelial cells expressed LPGDS. It is strongly expressed in brain pericytes following ischemic insult, suggesting that the ischemic pericytes are the major site of LPGDS expression following stroke. Knowledge of these expression profiles may be useful for future PC-based stem cell therapies.
2019年度